
Sperm Sample Preparation
Preparing the Sample

IMSI is an advanced form of intracytoplasmic sperm injection that uses high-magnification microscopy to examine sperm morphology in greater detail before selecting an individual sperm for injection into the egg. The technique is designed to help embryologists identify sperm with more favourable morphological characteristics for use during assisted reproductive treatment. IMSI provides an additional level of sperm assessment before the selected sperm is used for ICSI-based fertilisation. The procedure is performed in a specialised embryology laboratory using advanced microscopic equipment and requires careful handling of both sperm and eggs. Depending on the couple's fertility history, sperm parameters and treatment requirements, IMSI may be considered as part of an individualised IVF/ICSI treatment plan. The fertility specialist and embryology team determine whether this approach is appropriate based on the complete clinical evaluation.
During ICSI a single sperm is injected into the egg. But IMSI takes ICSI to the next level. IMSI allows us to choose the very best sperm using a high power microscope that usually magnifies image to 6000 times or more. At this magnification level, the embryologist can usually detect subtle structural alterations in sperm that a normal microscope could not detect. This helps our embryologist’s to choose a morphologically best sperm for injection.
IMSI procedure is carried out exactly like ICSI once the best sperm has been selected.
Studies suggest that IMSI offers no additional benefit over ICSI.
[box type=”info”] A well trained Embryologist can identify healthy sperms based on it’s swimming pattern even at a lower magnification
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IMSI involves detailed examination and selection of sperm under high magnification followed by injection of the selected sperm into an egg. The technique is performed in a specialised embryology laboratory.

Preparing the Sample

Detailed Morphological Assessment

Selecting the Suitable Sperm

ICSI-Based Fertilisation
Find answers about IMSI, sperm selection, the IMSI procedure, ICSI, treatment suitability and fertility outcomes.
IMSI stands for Intracytoplasmic Morphologically Selected Sperm Injection. It is an advanced sperm-selection technique that uses high-magnification microscopy to examine sperm morphology before selecting an individual sperm for injection into an egg.
ICSI involves injecting a selected sperm directly into an egg. IMSI adds a high-magnification sperm-selection step before the ICSI injection, allowing the embryologist to examine sperm morphology in greater detail.
During IMSI, individual sperm are examined under high magnification. The embryologist selects a sperm with suitable observed morphological characteristics for injection.
High magnification allows the embryologist to examine sperm morphology in greater detail than conventional microscopic assessment.
IMSI is not a separate IVF treatment by itself. It is an advanced sperm-selection technique that can be incorporated into an IVF/ICSI treatment cycle.
IMSI may be considered for selected couples where detailed sperm morphology assessment is considered clinically relevant. Suitability depends on the individual fertility evaluation.
No. IMSI is not necessarily required for every fertility treatment cycle. The fertility specialist and embryology team determine whether it is appropriate for an individual couple.
IMSI is intended to provide more detailed sperm selection, but treatment outcomes vary. The use of IMSI does not guarantee successful fertilisation or pregnancy.
No. Pregnancy depends on several factors including egg quality, sperm quality, embryo development, age and other reproductive factors.
When IMSI is performed as part of an IVF/ICSI cycle, eggs are generally collected through an egg retrieval procedure before the laboratory fertilisation process.
After selection, the chosen sperm is injected directly into the egg using an ICSI-based microinjection procedure.
After fertilisation, embryos are cultured and monitored in the embryology laboratory. Depending on the treatment plan, an appropriate embryo may later be transferred to the uterus or frozen for future use.
IMSI may be considered in selected cases involving male-factor fertility concerns, particularly when detailed sperm morphology assessment is considered useful.
The IMSI sperm-selection and injection procedures are laboratory procedures performed on sperm and eggs. The physical experience for the patient is mainly related to the other procedures involved in the IVF/ICSI treatment cycle, such as ovarian stimulation and egg retrieval.
The timing depends on the laboratory workflow, number of eggs and sperm requiring assessment, and the overall IVF/ICSI treatment protocol.
IMSI involves laboratory sperm selection and ICSI-based injection. The overall treatment risks are primarily related to the assisted reproductive treatment cycle, and individual risks should be discussed with the fertility specialist.
The cost varies between fertility centres and depends on whether IMSI is included in an IVF/ICSI package or charged as an additional laboratory service.
Yes. IMSI can be incorporated into an IVF/ICSI treatment cycle, and additional procedures may be considered according to the couple's fertility requirements.
The choice should be made with the fertility specialist and embryology team after reviewing sperm parameters, fertility history and the overall treatment plan.
Treatment outcomes can be influenced by sperm quality, egg quality, female partner's age, embryo development, reproductive health and other individual fertility factors.
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