Low Sperm Motility
When many sperm are not moving, vitality testing helps clarify whether they are alive.

Sperm vitality testing checks how many sperm in a semen sample are alive. A sperm can be alive even when it is not moving, so vitality assessment provides information that cannot be determined from motility alone. The Hypo-Osmotic Swelling (HOS) test evaluates the functional condition of the sperm cell membrane by observing how sperm respond when exposed to a specially prepared solution. This helps distinguish living, non-moving sperm from sperm that are no longer viable. The results are interpreted together with semen analysis findings such as sperm count, motility and morphology to provide a more complete understanding of sperm quality. HOS testing may be considered in selected cases where sperm movement is very low or when it is important to identify viable non-moving sperm for further fertility treatment planning.
HOS testing can provide additional information when sperm are not moving or when routine semen findings need further clarification.
When many sperm are not moving, vitality testing helps clarify whether they are alive.
A sample with no visible movement may still contain living sperm. Further assessment helps guide the next step.
Vitality adds context to a semen report. Your history and sample collection details also matter.
A modified HOS method may help select living nonmoving sperm for ICSI when clinically appropriate.
Follow the laboratory collection instructions carefully so the sample can be handled and assessed appropriately.
Usually, avoid ejaculation for 2 to 7 days. Follow the interval given by the laboratory.
Collect the full sample by masturbation. Avoid ordinary condoms and unapproved lubricants.
Report any sample loss, collection time, recent fever and medicines. Follow transport instructions.
HOS is a laboratory test of sperm membrane function. Its findings should be interpreted in the context of the complete semen report and treatment plan.

HOS findings should be reviewed with the fertility specialist or embryology team when clinically appropriate.
HOS provides additional information about sperm membrane function but does not replace routine semen analysis.
A sperm can be alive even when it is not moving, which is why vitality testing can add useful information.
Sample collection and handling details should be considered when interpreting semen findings.
HOS does not directly test sperm DNA or chromosomes and does not itself determine the ability to achieve pregnancy.
A vitality result should not be used as a standalone diagnosis of male fertility.
The current cost should be confirmed directly with the clinic.
HOS is a laboratory test performed on a semen sample.
Semen analysis and HOS testing may have separate charges.
The exact current cost should be confirmed with the clinic before booking.
Important to know
The provided page design does not list a fixed price. Ask the clinic for the current HOS test cost.
Cost Reference
Sperm Vitality / HOS Test: Confirm current pricing with Indore Infertility Clinic.
Find answers to common questions about sperm vitality, HOS testing, nonmoving sperm and sperm membrane function.
No. Some sperm are alive but do not move. Vitality testing helps distinguish living nonmoving sperm from dead sperm.
No. Sperm count measures how many sperm are present. HOS assesses sperm membrane function.
No. DNA fragmentation tests assess DNA damage. HOS measures a different sperm property.
The test is performed on a semen sample in the laboratory. It does not require a procedure on your body.
No. It is used when the semen findings or treatment plan make vitality assessment useful.
In selected cases, a modified HOS method can assist selection. The embryologist decides which method is appropriate.
Sometimes. Semen findings vary, and collection or handling can affect the result.
No. A single result cannot answer that. A specialist reviews the full findings and treatment options.
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